How to Prepare 0.1 M Ascorbic Acid Solution
Ascorbic acid, also known as vitamin C, is a crucial nutrient that plays a vital role in various biological processes. It is commonly used in pharmaceuticals, food preservation, and skincare products. To prepare a 0.1 M ascorbic acid solution, follow these simple steps:
1. Gather the necessary materials: Before starting, ensure you have all the required materials, including ascorbic acid powder, distilled water, a graduated cylinder, a beaker, a stirrer, and a label.
2. Weigh the ascorbic acid: Using a balance, weigh out 1.33 grams of ascorbic acid powder. This amount corresponds to the molar mass of ascorbic acid (C6H8O6), which is 176.12 g/mol. For a 0.1 M solution, you need 0.1 moles of ascorbic acid.
3. Dissolve the ascorbic acid: Pour 10 ml of distilled water into a beaker. Add the weighed ascorbic acid powder to the beaker and stir until the powder is completely dissolved. It may take a few minutes for the ascorbic acid to dissolve completely, especially if the water is cold.
4. Transfer the solution: Once the ascorbic acid is dissolved, carefully transfer the solution to a graduated cylinder. Make sure to use a pipette or dropper to avoid spills.
5. Add more water: Add distilled water to the graduated cylinder until the solution reaches the 100 ml mark. Be precise with the measurements to ensure the correct concentration.
6. Stir the solution: Use a stirrer to mix the solution thoroughly. It is essential to ensure that the ascorbic acid is evenly distributed throughout the solution.
7. Label the solution: Once the solution is prepared, label the bottle with the concentration (0.1 M), the date, and any other relevant information.
8. Store the solution: Store the ascorbic acid solution in a cool, dark place to maintain its stability. Keep it away from direct sunlight and extreme temperatures.
By following these steps, you can successfully prepare a 0.1 M ascorbic acid solution. This solution can be used for various applications, such as in experiments, skincare products, or food preservation. Always handle ascorbic acid with care, as it can be irritating to the skin and eyes.
